Repbase Reports |
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| 2008, Volume 8, Issue 7 |
| July 31, 2008 |
| Copyright © 2001-2024 - Genetic Information Research Institute, California |
| ISSN# 1534-830X |
| Page 750 |
EnSpm-2_DR |
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EnSpm-2_DR is an autonomous DNA transposon - a consensus. |
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Submitted: 31-Jul-2008 |
Accepted: 31-JUL-2008 |
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Key Words: EnSpm/CACTA; DNA transposon; Transposable Element; EnSpm; Autonomous DNA transposon; EnSpm-2_DR |
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Source: Danio rerio |
Organism: Danio rerio |
Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Cyprinidae; Danio |
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Authors: Kapitonov,V.V. and Jurka,J. |
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Title: Zebrafish En/Spm DNA transposons. |
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Journal: Repbase Reports 8(7), 750-750 (2008) |
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Abstract: EnSpm-2_DR is young family of autonomous En/Spm DNA transposons. The consensus sequence was derived based on multiple alignment of several copies of EnSpm-2_DR that are less then 5% divergent from each other. The consensus sequence codes for a 815-aa En/Spm transposase. Based on multiple alignment of diverse En/Spm transposases from plants, fungi and animals, we suggest that the En/Spm catalytic core is defined by a DDE triad. Its coordinates in EnSpm-2_DR transposase are defined by the 380-469-634 aa positions. Zebrafish EnSpm transposase contains also in its N-terminal portion a novel type of zinc finger motif, called ENS-finger: C-x2-C-x12-H-x4-H-x11-C-x4-C-x12-H-x4-H. EnSpm-2_DR transposons are characterized by 2-bp target-site duplications (TA - most frequent, CA, TG) and imperfect 30-bp terminal inverted repeats (6 mismatches). EnSpm-2_DR is a composite transposon: its 869-1079 portion is similar to the 172-388 portion of the TDR15 transposon. Contrary to plant En/Spm transposons with the canonical CACTA 5'-termini, zebrafish En/Spm transposons contain the CACAG or CACTG 5'-termini. Zebrafish En/Spm transposons differ also from their plant relatives by a target-site duplication length: it is 2-bp in zebrafish (3-bp in plants). This sequence was derived from sequence data generated by the Danio rerio Sequencing Group at the Sanger Institute.
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Derived: [1] (Consensus) |
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Download Sequence - Format: IG, EMBL, FASTA |
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References: |
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